Position located in Rochester, MN

Responsibilities of the Scientist – Immuno-oncology:

• Design and perform independent experiments in tissue culture, and viral infections in rodent tumor models. Execute experiments from set up to take down, and downstream cellular analysis.
• The position will play a critical role in the generation of new vector constructs and troubleshooting the vector expression in CAR T cells.
• Design, generate and characterize CAR or other constructs for genetically modified T cells.
• Independently design and lead pre-clinical in life studies including downstream analysis.
• Collect, analyze, and present scientific data to the research and management teams; with proposed next steps.
• Self-motivated and driven to work independently and collaboratively as part of a team.
• Coordinate research efforts and make key scientific and technical contributions for the strategic development of cross-functional projects.
• The candidate will perform independent experimental research but will work in close collaboration with the rest of the scientific team in a highly cross-functional and project-oriented environment.

Requirements of the Scientist – Immuno-oncology:

• PhD or Master degree in Immunology or a related field with a minimum of 3 years of industry or academic experience in immunology and in vivo oncology or pharmacology.
• Exceptional laboratory skills, with demonstrated proficiency in the development and execution of primary immune cell assays (T cell and/or myeloid cell).
• Deep understanding of adaptive immunity including T cell, B cell, myeloid or dendritic cell biology.
• Working knowledge of all stages of CAR design and virus generation.

Valued Complementary Skills: of the Scientist – Immuno-oncology:

• T cell activation assay (Mouse or human)
• T cell memory response recall assays: ELISPOT
• Immunohenotyping: Multiplex and tetramer analysis
• Immuno histochemistry, with focus on T cell markers (Multiplex)
• RNAscope
• Handling and processing human PBMC samples
• Processing of mouse spleen, Lymph nodes for downstream analysis
• Additional preferred qualifications: RNAseq analysis, Exome sequencing